Alpha Diagnostic International, Inc. Mouse/human beta-Defensin 3 control/blocking peptide #1 MBD31-P

Mouse/human beta-Defensin 3 control/blocking peptide #1 General Information The genes encoding human a and ß -defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated a ? and ß - based on distinctive, although similar, tri-disulfide linkages in the peptides. -def SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Mouse Form Antigen/blocking Peptide Antigen Human beta-defensin-3 is produced from 67 aa peptide precursor (mature peptide 23-67 aa). Mouse beta-defensin-3 is produced from a 63 aa peptide precursor (mature peptide 23-63 aa). A 13 aa peptide mapping at the N-terminus of mature mouse defensin-3 and a 15-aa peptide mapping at the N-terminus of mature human defensin-3. The two peptide were mixed (designated MBD31-P; control peptides) SpCrossreactivity The mouse and human defensin-3 peptides (MBD31-P) used for antibody (MBD31-S or MBD31-A) production are specific for mouse and human defensin-3. The resulting antibodies reacts with both mouse and human defensin-3 but not other beta-defensins. The MBD31-P control peptide is available for antibody blocking to confirm specificity of antibodies. It is not suitable for testing by Western due to small size. Antibody cross-reactivity in various species has not been studied For research use only, not for diagnostic or therapeutic use.