Alpha Diagnostic International, Inc. Mouse/Human Hepcidin-25 control/blocking peptide # 3 HEPC13-P

Mouse/Human Hepcidin-25 control/blocking peptide # 3 General Information Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Mouse/Human Form Antigen/blocking Peptide Antigen A 7-aa peptide sequences from the N-terminus of mature human and mouse 25-aa HEPC were synthesized. The two peptides (designated HEPC13-P or control peptide) conjugated to KLH SpCrossreactivity The mouse HEPC13-P sequences from mouse/rat, and human differ by just aa in 25-aa mature HEPC. The HEPC13-P sequence is truncated by 5-aa in the 20-aa major secreted HEPC. Anti-HEPC13-P do not have significant reactivity with 20-aa HEPC peptide. Anti-HEPC13-P could potentially react with the unprocessed larger 60-aa pro-HEPC if present in the samples or tissues. Antibody cross-reactivity in various species has not been studied. Full length, human 25-aa (cat # HEPC61-P) and 20-aa HEPC (cat # HEPC71-P) and mouse 25-aa HEPC (cat # HEPC-81) are also available for various studies For research use only, not for diagnostic or therapeutic use.