Alpha Diagnostic International, Inc. Monoclonal Anti-Glutathione Transferase (GST, E. coli) IgG-Biotinylated GST16-BTN

Monoclonal Anti-Glutathione Transferase (GST, E. coli) IgG-Biotinylated General Information Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified p SpeciesReactivity E. coli Form Antibody, purified Antigen Bacterial GST (Schistosoma japonicum, ~27 kda) was expressed in E. coli and purified (>97%). SpCrossreactivity The antibodies have been tested by ELISA (suggested dilution 1K-10K) using 50-100 ng/well GST-coated ELISA plates. They recognize both native GST or GST fusion proteins in Western blots (suggested dilution 1K). Antibody dilutions will vary depending upon the type of technique used to detect primary antibody such calorimetric or chemiluminescence substrates. The nature and concentration of GST or GST fusion proteins may also affect antibody concentration. For research use only, not for diagnostic or therapeutic use.