Alpha Diagnostic International, Inc. Recombinant purified FLAG tag-Protein (~9 kda) control WB +ve control FLAG12-C

Recombinant purified FLAG tag-Protein (~9 kda) control WB +ve control General Information Recombinant DNA technology allows the addition of short pieces of well-defined tags, ?peptides? or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a sel SpeciesReactivity E. coli Form Protein Control Antigen An 8-aa peptide sequence (DYK DDD DK) was expressed as N-terminal fusion protein of ~10 kDa in E. coli and purified to >95%. It is supplied for use in Western blot (Cat # FLAG12-C) or for ELISA coating or other applications (Cat # FLAG15-R). #FLAG12-C protein for Western blot +ve control (Cat # FLAG12-C) is supplied in SDS-PAGE sample buffer (reduced). Load 10 ul/lane of FLAG12-C for good visibility with appropriate ant-Flag antibodies. The FLAG12-C protein migrate as ~9-10 kda band. It will be necessary to have proper size gel (e.g., 10% or 4-10% for proper resolution of small protein For research use only, not for diagnostic or therapeutic use.