Alpha Diagnostic International, Inc. Human/Rat Estrogen Receptor-beta (ER-b) 1/2 Control/blocking peptide # 5 ERB15-P

Human/Rat Estrogen Receptor-beta (ER-b) 1/2 Control/blocking peptide # 5 General Information Rat/mouse ER 1 gene encodes a protein of 485 aa with a calculated size of approx. 54 kDa. ER 1 is 477 aa in human. As compared to ER , ER is highly conserved in the DNA-binding domain (>90% homology) and the C-terminal ligand binding domain (55% homolog SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Human Form Antigen/blocking Peptide Antigen 18-aa peptide of Human/Rat ER 1; Designated (ERB15-P or control peptide) conjugated to KLH; epitope location ~ N-terminal, modulating domain SpCrossreactivity ERb15-P peptide sequence is 100% identical in mouse, rat, human, monkey, 94% in ovine, pig, canine, and 88% in bovine ER 1. The sequence is also conserved in rat ER 2 and the variants of ER 1/ 2 (ER 1 3 & ER 2 3). It is not clear, at present, if all of these ER isoforms can be seen and distinguished by this antibody because of very small size differences in various ER isoforms. Antibody crossreactivity in various species and isoform reactivity has not been established For research use only, not for diagnostic or therapeutic use.