Alpha Diagnostic International, Inc. Anti-Human Estrogen Receptor-beta (ER-b) 1/2 IgG # 4, aff pure ERB14-A

Anti-Human Estrogen Receptor-beta (ER-b) 1/2 IgG # 4, aff pure General Information Rat/mouse ER 1 gene encodes a protein of 485 aa with a calculated size of approx. 54 kDa. ER 1 is 477 aa in human. As compared to ER , ER is highly conserved in the DNA-binding domain (>90% homology) and the C-terminal ligand binding domain (55% homolog SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Human SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen 20-aa peptide of Human ER ; Designated (ERB14-P or control peptide) conjugated to KLH; epitope location ~ N-terminus SpCrossreactivity The 20-aa human ERb14-P immunogenic peptide shows 84% homology with the rat ER 1 (1-3).. The sequence is also conserved in rat ER 2 and the variants of ER 1/ 2 (ER 1 3 & ER 2 3). It is not clear, at present, if all of these ER isoforms can be seen and distinguished by this antibody because of very small size differences in various ER isoforms. Actual crossreactivity of antibodies in all species is not established For research use only, not for diagnostic or therapeutic use.