Alpha Diagnostic International, Inc. Human eNOS cDNA probe eNOS51-D-2

Human eNOS cDNA probe General Information Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-II SpeciesReactivity Human Form PCR Ready Antigen The cDNA probe is provided as a double stranded cDNA fragment as lyophilized powder. It can be re-suspended in a desired buffer (50-100ul buffer: 10mM Tris-HCl, pH 7.5, 0.1 mM EDTA). Store is suitable size aliquots at ?20oC for 6-12 months. The probe has 5' overhangs and >98 % purity. The length of the probe is derived from a segment of human endothelial constitutive nitric oxide synthase (eNOS) gene, which has a length of approximately 1.23 kbp. The probe may be labeled by radioactive or non-radioactive compounds using either random primer labeling or nick translation. SpCrossreactivity The cDNA probe is provided as a double stranded cDNA fragment as lyophilized powder. It can be re-suspended in a desired buffer (50-100ul buffer: 10mM Tris-HCl, pH 7.5, 0.1 mM EDTA). Store is suitable size aliquots at ?20oC for 6-12 months. The probe has 5' overhangs and >98 % purity. The length of the probe is derived from a segment of human endothelial constitutive nitric oxide synthase (eNOS) gene, which has a length of approximately 1.23 kbp. The probe may be labeled by radioactive or non-radioactive compounds using either random primer labeling or nick translation. For research use only, not for diagnostic or therapeutic use.