Alpha Diagnostic International, Inc. Bovine Endothelial Nitric Oxide Synthase (eNOS/NOS-III) Control/blocking peptide # 2 eNOS41-P

Bovine Endothelial Nitric Oxide Synthase (eNOS/NOS-III) Control/blocking peptide # 2 General Information Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-II SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Bovine Form Antigen/blocking Peptide Antigen A synthetic peptide (1185-1205) of bovine eNOS (protein accession #P29473, refs 1); Designated (eNOS41-P or control peptide) coupled to KLH; Epitope location~ C-terminal SpCrossreactivity Bovine eNOS41-P peptide sequence is 100% conserved in rabbit, pig, sheep, 93% in canine, chicken, human, and Chimp, 87% in mouse, 85% in rat NOS-3/eNOS/NOS-III proteins. No significant sequence homology of eNOS41-P is seen with NOS-1/NOS-2 or other proteins. Antibodies react with mouse, rat, human and human eNOS. Antibody crossreactivity in various species is not established. For research use only, not for diagnostic or therapeutic use.