Alpha Diagnostic International, Inc. Mouse Monoclonal Anti-Bovine Endothelial Nitric Oxide Synthase (eNOS/NOS-III) antibody #4 eNOS41-M

Mouse Monoclonal Anti-Bovine Endothelial Nitric Oxide Synthase (eNOS/NOS-III) antibody #4 General Information Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-II SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2486-seconda ry-antibodies-mouse. htm SpeciesReactivity Mouse SugNegativeControl Non-immune Rabbit IgG Cat# 20008-1 Form Mono-ascites SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen A synthetic peptide (1185-1205) of bovine eNOS (protein accession #P29473, refs 1); Designated (eNOS41-P or control peptide) coupled to KLH; Epitope location~ C-terminal SpCrossreactivity Bovine eNOS41-P peptide sequence is 100% conserved in rabbit, pig, sheep, 93% in canine, chicken, human, and Chimp, 87% in mouse, 85% in rat NOS-3/eNOS/NOS-III proteins. No significant sequence homology of eNOS41-P is seen with NOS-1/NOS-2 or other proteins. Antibodies react with mouse, rat, human and human eNOS. Antibody crossreactivity in various species is not established. For research use only, not for diagnostic or therapeutic use.