Alpha Diagnostic International, Inc. Mouse Cyclooxygenase 3 (Cox-3) Control/blocking peptide # 1 COX31-P

Mouse Cyclooxygenase 3 (Cox-3) Control/blocking peptide # 1 General Information COX3 and PCOX1a are made from the COX1 gene but retain intron 1 in their mRNAs. PCOX-1b (53 kDa) lacks the intron 1. This intron introduces an insertion of 30-34aa, depending on mammalian species, into hydrophobic signal peptide that directs COX1 into th SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Mouse Form Antigen/blocking Peptide Antigen 12aa peptide (1-12aa) of Mouse COX3 (1); Designated (COX31-P or control peptide) conjugated to KLH.; epitope location ~ N-terminus SpCrossreactivity COX31-P control peptide is 100% conserved in Human and dog COX3 and PCOX1a proteins. Antibody (cat # COX31-A) cross-reactivity in various species is not known. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity (see detailed protocol at the web site).). For research use only, not for diagnostic or therapeutic use.