Alpha Diagnostic International, Inc. Anti-Mouse Cyclooxygenase 3 (Cox-3) IgG # 1, aff pure COX31-A

Anti-Mouse Cyclooxygenase 3 (Cox-3) IgG # 1, aff pure General Information COX3 and PCOX1a are made from the COX1 gene but retain intron 1 in their mRNAs. PCOX-1b (53 kDa) lacks the intron 1. This intron introduces an insertion of 30-34aa, depending on mammalian species, into hydrophobic signal peptide that directs COX1 into th SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2479-seconda ry-antibodies-goat.h tm SpeciesReactivity Mouse SugNegativeControl Non-immune Goat IgG Cat# 20011-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen 12aa peptide (1-12aa) of Mouse COX3 (1); Designated (COX31-P or control peptide) conjugated to KLH.; epitope location ~ N-terminus SpCrossreactivity COX31-P control peptide is 100% conserved in Human and dog COX3 and PCOX1a proteins. Antibody (cat # COX31-A) cross-reactivity in various species is not known. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity (see detailed protocol at the web site).). For research use only, not for diagnostic or therapeutic use.