Alpha Diagnostic International, Inc. Human Calcitonin Gene Related Peptide (CGRP) Control/blocking peptide #2 CGRP12-P

Human Calcitonin Gene Related Peptide (CGRP) Control/blocking peptide #2 General Information The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32 aa peptide found in the parafollicular ?C? cells of the thyroid in mammals. I SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Human Form Antigen/blocking Peptide Antigen 19-aa peptide of Human CGRP/CALCA (protein accession # P06881, refs 1; Designated (CGRP12-P or control peptide) conjugated to KLH, Location ~C-terminus SpCrossreactivity The human CGRP12-P is quite conserved in various species: canine (100%), bovine, sheep, rat , chicken, (92%), frog and pig (85%). The antibody has also detected hamster CGRP (2). There are just 2 aa change between the human CGRP1 and CGRP2. However, no significant sequence homology is detected with amylin, calcitonin or adrenomedullin. Antibody cross-reactivity in various species has not been studied. The CGRP12-P control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. For research use only, not for diagnostic or therapeutic use.