Alpha Diagnostic International, Inc. Anti-Mouse 11-beta hydroxysteroid dehydrogenase-2 (11 beta-HSD-2) IgG #2, aff pure BHSD22-A

Anti-Mouse 11-beta hydroxysteroid dehydrogenase-2 (11 beta-HSD-2) IgG #2, aff pure General Information 11 HSD-2 (rat 400-aa, mouse 396-aa, human 405-aa) is a ~41 kDa glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11 -HSD2 are in the lumen and cytoplasm of ER, respectively. 11 -HS SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Mouse SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen A 16-aa peptide (designated BHSD22-P or control peptide) Gene Accession # P51661, mapping near the C-terminus of Mouse 11 -HSD2 (1) synthesized, coupled to KLH, and polyclonal antibodies generated in rabbits. ;Epitope location ~ Mid-region SpCrossreactivity The mouse BHSD22-P peptide is not well conserved in rat and human 11 -HSD2. We recommend the use of cat # BHD21-A and BHD23-A for rat and human 11 -HSD2, respectively. No significant sequence homology of BHSD22-P is seen with 11 -HSD1 or other proteins. For research use only, not for diagnostic or therapeutic use.