Alpha Diagnostic International, Inc. Human ATII, Type 2 receptor Control/blocking peptide # 2 AT22-P

Human ATII, Type 2 receptor Control/blocking peptide # 2 General Information Angiotensin II, interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). However, major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359 aa; AT1B/AT III, 359 aa; and AT1 SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Human Form Antigen/blocking Peptide Antigen 18-aa peptide of human AT II type2 (gene accession # P50052); Designated (AT21-P or control peptide) conjugated to KLH SpCrossreactivity The 18 AA human AT21-P immunogenic peptide is 100% homologous in rabbit, 88% identical in rat and 83% with mouse AT2 receptors. It has no sequence homology with AtII type 1 or other G-protein coupled receptors. The antibodies have been shown to be specific for AtII, type 2 as shown by the expression of the ATII, type 1 and 2 and detection with antibodies (see refs 2). Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.