Alpha Diagnostic International, Inc. Rat ATII, Type 1 receptor Control/blocking peptide # 1 AT11-P

Rat ATII, Type 1 receptor Control/blocking peptide # 1 General Information Angiotensin II, interacts with two types of G-protein coupled membrane receptors, AT1 (type 1) and AT2 (type 2). However, major cardiovascular effects appear to be mediated through AT1. AT1 has three isoforms (rat AT1A 359 aa; AT1B/AT III, 359 aa; and AT1 SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Rat Form Antigen/blocking Peptide Antigen 10-aa peptide of rat AT II type 1 gene accession # P25095, refs 1); Designated (AT11-P or control peptide); ~N-terminal, Extracellular SpCrossreactivity The 10 AA rat AT11-P immunogenic peptide is 100% identical in mouse, rat, human, ovine, pig, dog, chimpanzee and rabbit. The sequence is the same in all AT II receptor isoforms (Type 1a, 1b, and 1c). It has no sequence homology with other G-protein coupled receptors. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.