Alpha Diagnostic International, Inc. Rat AMP Activated Kinase-a2 (AMPK-a2) Control/blocking peptide AMPKA21-P

Rat AMP Activated Kinase-a2 (AMPK-a2) Control/blocking peptide General Information AMPK is a heterotrimer of a catalytic subunit (~63 kDa), and two non-catalytic subunits, (~40 kDa) and (~38 kDa). These subunits exist in multiple isoforms ( 1, 2, 1, 2, 1 and 2). Coexpression of all three subunit is required for kinase activ SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Rat Form Antigen/blocking Peptide Antigen 20aa peptide of rat AMPK- 2 (gene accession # Q09137 AAK2); Designated (AMPKA21-P) Control/blocking peptide) conjugated to KLH SpCrossreactivity The rat AMPKA21-P peptide is 85% conserved in human, pig and 90% in mouse AMPK-a2. No significant sequence homology of AMPKA21-P is seen with AMPK-a1 or other proteins. Antibody reactivity in various species is not known. The AMPKA21-P, Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.