Alpha Diagnostic International, Inc. Human Angiotensin converting enzyme 1 (ACE-1) control peptide # 2 ACE12-P

Human Angiotensin converting enzyme 1 (ACE-1) control peptide # 2 General Information ACE (also known as dipeptidyl carboxypeptidase-1, DCP1; Kininase-II, ACE1) gene has been mapped at human chromosome 17q23. The s-ACE and t-ACE isoforms are generated by alternative splicing of ACE-2 gene. Somatic-ACE, a Zn (II) containing dipeptidyl carbo SugApplications Control or blocking peptides are recommended for ELISA plate coating for direct ELISA testing of antibodies or competitive ELISA, and antibody blocking or neutralization. SpeciesReactivity Human Form Antigen/blocking Peptide Antigen 14-aa peptide of Human testicular-ACE (gene accession # P22966, refs 1); Designated (ACE12-P or control peptide); location: ~N-terminal, Extracellular SpCrossreactivity Human testicular ACE12-P control peptide is 92% conserved in mouse and 57% in rat t-ACE. ACE12-P epitope is located near the N-terminus that is found in membrane bound as well soluble t-ace. So the antibodies are expected to react with both isoforms. No significant sequence homology exist with the s-Ace of any species. Antibody cross-reactivity in various species is not known. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.