Alpha Diagnostic International, Inc. Anti-rat ATP binding cassette, subfamily G, member 8, (ABCG8) IgG # 1, aff pure ABCG81-A

Anti-rat ATP binding cassette, subfamily G, member 8, (ABCG8) IgG # 1, aff pure General Information Rat ABCG8 protein is alternatively spliced: A full length isoforms 3 (694-aa), isoforms 2 (671-aa; missing 56-77 aa, and 398-aa); isoforms 1 (672aa, missing 56-77 aa). Actual expression of various protein isoforms and their physiological relevance is n SugApplications Most Antibodies are tested by ELISA and Western. Additional applications, such as IHC or IP may have been tested and will be listed in the product data sheets or published research papers using our products. Typically, antibodies are tested against the SugSecAntibodies http://4adi.com/comm erce/ccc2480-seconda ry-antibodies-rabbit .htm SpeciesReactivity Rat SugNegativeControl Non-immune Rabbit IgG Cat# 20009-1 Form Affinity purified IgG SugPositiveControl Recombinant or naturally purified proteins or cells of tissues expressing the protein under study can be used as positive controls for Western or IHC. Please review the ?General Information? tan under Product overview section. Control or blocking peptid Antigen 15-aa peptide from Rat ABCG8 (protein accession #P58248 refs 1); Designation (ABCG81-P, control or blocking peptide) SpCrossreactivity Rat ABCG81-P control peptide is 100% conserved in rat ABCG-8 isoforms 1-3. It is 65% conserved in mouse ABCG8 and no appreciable conservation in human ABCG8. No significant conservation of ABCG31-P is observed with other ABCGs (ABCG1-7) or other proteins. Antibody reactivity and actual expression of various protein isoforms and their physiological relevance is not understood. Antibody cross-reactivity in various species has not been studied. Control peptide, because of its low mol. Wt (<3 kDa), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity For research use only, not for diagnostic or therapeutic use.