Alpha Diagnostic International, Inc. Peptide/Protein conjugation to Pre-activated KLH Kit (sufficient for 4 reactions) 80250

Peptide/Protein conjugation to Pre-activated KLH Kit (sufficient for 4 reactions) General Information All small peptides/haptens (such as steroids or drugs or chemicals) must be coupled to a carrier protein (KLH or Keyhole limpet hemocyanin, BSA, Bovine serum albumin or Ovalbumin, etc) in order to elicit high titer antibodies. Generally, peptides can be c Form Kit Samples user supplied proteins, peptides or antibodies KitContents Kit Components 1. Pre-Activated BSA kit #80240 contains 1 mg x 4 vials, # 80241 2. Pre-Activated KLH kit # 80250 contains 1 mg x 4 vials, # 80251 3. Pre-Activated Ovalbumin kit # 80260 contains 1 mg x 4 vials, # 80261 4. Conjugation Buffer (20X), 25 ml; #CB-20 (common to kit# 80240, #80250, and 80260 kits). Dilute 1:20 with water. If buffer salts have forms crystals then they can be dissolved by warming or mixing at room temp for 1-2 hrs. Store kit at 4oC for 6-12 months. SpCrossreactivity General purpose preactivated carrier protein (KLH) for coupling peptide or other haptens to KLH. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.