Alpha Diagnostic International, Inc. Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP) 80225

Horseradish peroxidase (HRP) conjugation kit for antigen/antibodies (Micro; 3 x 200 ug HRP) General Information HRP is widely used in ELISA/EIA as a detection molecule. HRP, a glycoprotein (40 kDa), is available in highly purified form, relative inexpensive, very stable, and provides several options (free amino, carboxyl, and carbohydrate groups) for covalent coup Form Kit Samples user supplied proteins, peptides or antibodies KitContents 1. 3 visal of Preactivated HRP (200 ug; lyophilized) # 80226 2. Conjugation Buffer, 0.1 M NaHCO3, pH 9.5 (10 X), 100 ml; (dilute 1:10 with water before use) # 80222 3. Blocking Buffer; 500 ul; # 80223 4. Conjugate Stabilizer; 2 ml., # 80224 5. Dialysis bag (Cut off size 10-12 kda) # DB-10 If proteins of 10kda or less are being coupled then it is a good idea to use membranes with lower cut-off such as (3 kda). Wash the membrane thoroughly with 70% ethanol and then with distilled water. SpCrossreactivity General purpose reagents for coupling antibodies or proteins to HRP. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.