Alpha Diagnostic International, Inc. Rabbit IgG (total) ELISA Kit, 96 tests, Quantitative 6520

Rabbit IgG (total) ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Rabbit Form Stds=10-160 ng/ml ; Sample=100 ul; Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Rabbit IgG coated strip plate (8 wells x 12 strips) 6521 2. Rabbit IgG Std. A (0 ng/ml), 0.25 ml 65223. Rabbit IgG Std. B (100 ng/ml), 0.25 ml 65234. Rabbit IgG Std. C (300 ng/ml), 0. 25 ml 65245. Rabbit IgG Std. D (500 ng/ml), 0. 25 ml 65256. Rabbit IgG Std. E (800 ng/ml), 0. 25 ml 65267. Rabbit IgG Std. F (1200 ng/ml), 0. 25 ml 65278. (20x) Sample Diluent, 10 ml SD-20T9. (100x) Anti-Rabbit IgG-HRP Conjugate, 0.12 ml 652910. Wash Buffer (100x), 10ml WB-10011. TMB Substrate, 12 ml 8009112. Stop solution, 12 ml 8010113. Instruction Manual M-6520 SpCrossreactivity The antibodies used in this kit are specific to IgG and does not show any significant reactivity to rabbit IgA, IgM, or IgE or other serum proteins. The antibodies used in this kit are not specific for any IgG subtype (IgG1, IgG2, etc.). It will detect total IgGs (heavy chain specific). Rabbit IgG ELISA kit species crossreactivity was tested with the following animal serum (IgG) at dilutions of 1:5k: Mouse, Hamster, Goat, Guinea Pig, Rat, Human, Monkey, and Chicken serum IgG had no significant crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.