Alpha Diagnostic International, Inc. Rat IgM ELISA Kit, 96 tests, Quantitative 6480

Rat IgM ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Rat Form Stds=0-10,000 ng/ml ; Sample=20 ul; Sensitivity= 500 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Rat IgM coated strip plate (8 wells x 12 strips) 6481 2. Rat IgM Standard A (0 ug/ml), 0.25 ml 6482 3. Rat IgM Standard B (0.5 ug/ml), 0.25 ml 6483 4. Rat IgM Standard C (1 ug/ml), 0. 25 ml 6484 5. Rat IgM Standard D (2 ug/ml), 0. 25 ml 6485 6. Rat IgM Standard E (4 ug/ml), 0. 25 ml 6486 7. Rat IgM Standard F (10 ug/ml), 0. 25 ml 6487 8. Sample Diluent (20X), 10 ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. (100x )Anti- Rat IgM HRP Conjugate, 0.12 ml 6488 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6480 SpCrossreactivity The antibodies used in this kit are specific to IgM and does not show any significant reactivity to rat IgA, IgG, or IgE or other serum proteins. Rat IgM ELISA kit species crossreactivity was tested with the following animal serum (IgM) at dilutions of 1:500: Significant crossreactivity was observed with Mouse (65%), Human (27%), and Hamster (32%) serum IgM. Guinea Pig, Rabbit, Monkey, Bovine, FBS, Pig, and Chicken serum IgM had less than 1% crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.