Alpha Diagnostic International, Inc. Rat IgG (total) ELISA Kit, 96 tests, Quantitative 6420

Rat IgG (total) ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Rat Form Stds=10-200 ng/ml ; Sample=100 ul; Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Rat IgG coated strip plate (8 wells x 12 strips) 64212. Rat IgG Standard A (0 ng/ml), 0.25 ml 64223. Rat IgG Standard B (50 ng/ml), 0.25 ml 64234. Rat IgG Standard C (100 ng/ml), 0. 25 ml 64245. Rat IgG Standard D (250 ng/ml), 0. 25 ml 64256. Rat IgG Standard E (500 ng/ml), 0. 25 ml 64267. Rat IgG Standard F (1000 ng/ml), 0. 25 ml 64278. Sample Diluent (20X), 10 ml SD-20T9. Wash Buffer (100X), 10 ml WB-10010. (100x )Anti-Rat IgG-HRP Conjugate, 0.12 ml 642811. TMB Substrate, 12 ml 8009112. Stop solution, 12 ml 8010113. Instruction Manual M-6420 SpCrossreactivity The antibodies used in this kit are not specific for any IgG subtype (IgG1, IgG2, etc.). This kit is also specific to IgG and does not show any significant reactivity to Rat IgA, IgM, IgE or other serum proteins. Rat IgG ELISA kit species crossreactivity was tested with the following animal serum (IgG) at dilutions of 1:500: Mouse, Hamster, Goat, Guinea Pig, Rabbit, Human, Monkey, Bovine, FBS (fetal bovine serum) , Sheep, Pig, and Chicken serum IgG had less than 1% crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.