Alpha Diagnostic International, Inc. Mouse IgG2b ELISA Kit, 96 tests, Quantitative 6350

Mouse IgG2b ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 i SpeciesReactivity Mouse Form Stds=5-100 ng/ml; Sample=100 ul; Sensitivity= 2 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Mouse IgG2b coated strip plate (8 wells x 12 strips) 63512. Mouse IgG2b Std. A (0 ng/ml), 0.220 ml 63523. IgG Std B (25 ng/ml), 0.220 ml 63534. IgG Std C (50 ng/ml), 0. 220 ml 63545. IgG Std D (100 ng/ml), 0. 220 ml 63556. IgG Std E (250 ng/ml), 0. 220 ml 63567. IgG Std F (500 ng/ml), 0. 220 ml 63578. IgG Std G (1000 ng/ml), 0. 220 ml 63589. Sample Diluent (20X), 10 ml SD-20T10. Wash Buffer (100X), 10 ml WB-10011. 100x Anti-mouse IgG2b-HRP Conjugate,0.12 ml 635912. TMB Substrate, 12 ml 8009113. Stop solution, 12 ml 8010114. Instruction Manual M-6350 SpCrossreactivity The antibodies used in this kit are specific for IgG2b with no significant reactivity to IgG1, 2a-3 or IgA, IgM, or IgE. Mouse IgG2a ELISA kit species crossreactivity was tested with the following animal serum at dilutions of 1:500: No significant crossreactivity was observed with Rat, Hamster, Guinea Pig, Rabbit, Human, Monkey, Goat, Sheep, FBS, Bovine, Pig, and Chicken serum IgG2a. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.