Alpha Diagnostic International, Inc. Mouse IgG2a ELISA Kit, 96 tests, Quantitative 6340

Mouse IgG2a ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 i SpeciesReactivity Mouse Form Stds=20-400 ng/ml; Sample=100 ul (diluted); Sensitivity= 10 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Mouse IgG2a coated strip plate (8 wells x 12 strips) 6341 2. Mouse IgG2a Standard A (0 ng/ml), 0.22 ml 6342 3. Mouse IgG2a Standard B (100 ng/ml), 0.22 ml 6343 4. Mouse IgG2a Standard C (250 ng/ml), 0. 22 ml 6344 5. Mouse IgG2a Standard D (500 ng/ml), 0. 22 ml 6345 6. Mouse IgG2a Standard E (1000 ng/ml), 0. 22 ml 6346 7. Mouse IgG2a Standard F (2000 ng/ml), 0. 22 ml 6347 8. Sample Diluent (20X), 10 ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. Anti-mouse IgG2a-HRP Conjugate (100x), 0.12 ml 6349 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Instruction Manual M-6340 SpCrossreactivity The antibodies used in this kit are specific for IgG2a with no significant reactivity to IgG1, 2b-3 or IgA, IgM, or IgE. Mouse IgG2a ELISA kit species crossreactivity was tested with the following animal serum at dilutions of 1:500: No significant crossreactivity was observed with Rat, Hamster, Guinea Pig, Rabbit, Human, Monkey, Goat, Sheep, FBS, Bovine, Pig, and Chicken serum IgG2a. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.