Alpha Diagnostic International, Inc. Mouse IgG (total) ELISA Kit, 96 tests, Quantitative 6320

Mouse IgG (total) ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 i SpeciesReactivity Mouse Form Stds=10-200 ng/ml; Sample=100 ul (diluted); Sensitivity= 10 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Mouse IgG coated strip plate (8 wells x 12 strips) 6321 2. Mouse IgG Std. A (0 ng/ml), 0.25 ml 6322 3. Mouse IgG Std. B (50 ng/ml), 0. 25 ml 6323 4. Mouse IgG Std. C (100 ng/ml), 0. 25 ml 6324 5. Mouse IgG Std. D (300 ng/ml), 0. 25 ml 6325 6. Mouse IgG Std. E (600 ng/ml), 0. 25 ml 6326 7. Mouse IgG Std. F (1000 ng/ml), 0. 25 ml 6327 8. 20x Sample Diluent, 10ml SD-20 9. Wash Buffer (100X), 10 ml WB-100 10. HRP conjugate diluent, 12ml HD-1 11. 100x Anti-mouse IgG-HRP Conjugate, 0.12 ml 6328 12. TMB Substrate, 12 ml 80091 13. Stop solution, 12 ml 80101 14. Instruction Manual M-6320 SpCrossreactivity The antibodies used in this kit are not specific for any IgG subtype (IgG1, IgG2, etc.). This kit is also specific to IgG and does not show any significant reactivity to mouse IgA, IgM, IgE or other serum proteins. Mouse IgG ELISA kit species crossreactivity was tested with the following animal serum at dilutions of 1:4k: Significant crossreactivity was observed with rat (16%), and hamster (3%) serum. Guinea Pig, Rabbit, FBS, Bovine, Goat, Sheep, Pig, Human, Monkey, and Chicken serum IgG had less than 1% crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.