Alpha Diagnostic International, Inc. Mouse RF Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative 6200

Mouse RF Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative General Information Rheumatoid Factors (RFs) were described as Immunoglobulins (IgGs) in rheumatoid arthritis as detected by agglutination of rabbit IgG coated erythrocytes (1). It was later demonstrated that RFs were IgM molecules reactive with the Fc-portion of the IgG fr SpeciesReactivity Mouse Form Stds=-ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified RF Coated Microwells (96 wells) 6201 2. Mouse Anti-RF negative serum, control 1 ml 6202 3. Mouse Anti-RF positive serum, control 1 ml 6203 4. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 6204 5. Sample Diluent(10X), 10ml SD-10 6. Wash Solution (100X), 10 ml WB-100 7. TMB Substrate Solution, 12 ml 80091 8. Stop Solution, 12 ml 80101 9. Complete Instruction Manual M-6200 SpCrossreactivity Mouse Total IgG RF is not specific and this kit will detect RFs of IgG, IgA, and IgM origins. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.