Alpha Diagnostic International, Inc. Mouse anti-Scl70 Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative 6110

Mouse anti-Scl70 Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are SpeciesReactivity Mouse Form Stds= 0-250 ng/ml; Sample: 100 ul (1:100); Sensitivity; 10 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Scl-70 Coated Microwells (96 wells) 6111 2. Mouse Ig?s std A (0 ng/ml), 0.750 ml 6112 3. Mouse Ig?s std B (62.5 ng/ml), 0.750 ml 6113 4. Mouse Ig?s std C (125 ng/ml), 0.750 ml 6 1 1 4 5. Mouse Ig?s std D (250 ng/ml), 0.750 ml 6 1 1 5 6. Mouse Ig?s std E (500 ng/ml), 0.750 ml 6 1 1 6 7. Mouse Ig?s std F (1000 ng/ml), 0.750 ml 6 1 1 7 8. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.12 ml 6 1 1 8 9. Sample Diluent (10X), 10ml SD-10 10. Wash solution (100X), 10 ml WB-100 11. TMB Substrate solution,12 ml. 8 0 0 9 1 12. Stop solution,12 ml. 8 0 1 0 1 13. Complete Instruction Manual. M - 6 1 1 0 SpCrossreactivity Scl-70-1 coating antigen has been immunoaffinity purified from rabbit/calf thymus using anti-Scl-70-1 sepharose. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with the ELISA. Homogeneity of Scl-70 is also verified by SDS-PAGE. This kit measures anti-Scl70 Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.