Alpha Diagnostic International, Inc. Discontinued Alternate Cat#6110, Mouse Anti-Scl70 Ig's (total (A+G+M) ELISA kit, Qualitative 6100

Discontinued Alternate Cat#6110, Mouse Anti-Scl70 Ig's (total (A+G+M) ELISA kit, Qualitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Antibody to Scl-70 antigen was originally called Scl-1. Anti-Scl-70 has been shown to react with a cellular antigen known as DNA topoisomerase I, which is responsible for the relaxation of supercoiled DNA. Anti-Scl-70 is found in ~75% patients with the diffuse progressive form of scleroderma. The presence of anti-Scl-70 seems to exclude the presence of anti-centromere antibody, which is a marker for a subset of patients with scleroderma know as CREST (calcinosis, sclerodactyly, and telangiectasia) syndrome. The frequency of ANA positivity in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren?s syndrome (SS). Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis, and immunoblotting are also used to define antibody specificity. Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases. ADI?s Anti-Scl-70 ELISA, has been developed to screen the presence of anti-Scl-70 in mouse serum. SpeciesReactivity Mouse Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Scl-70 Coated Microwells (96 wells) 61012. Mouse Anti-Scl-70 negative serum, Control 1ml 61023. Mouse Anti-Scl-70 positive serum, control 1ml 61034. Goat Anti-mouse IgG (H+L)-HRP, Conjugate (100X), 0.2 ml 61045. Sample Diluent (10X), 10ml SD-106. Wash Solution (100X), 10 ml WB-1007. TMB Substrate Solution, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual, Cat. M-6100 M-6100 Form Stds=-ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests SpCrossreactivity Scl-70-1 coating antigen has been immunoaffinity purified from rabbit/calf thymus using anti-Scl-70-1 sepharose. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with the ELISA. Homogeneity of Scl-70 is also verified by SDS-PAGE. This kit detects anti-Scl70 Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as an..