Alpha Diagnostic International, Inc. Chicken IgG ELISA Kit, 96 tests, Quantitative 6020

Chicken IgG ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Chicken Form Stds=0-1000 ng/ml ; Sample=20 ul; Sensitivity= 15 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Chicken IgY (IgG) coated strip plate (8 wells x 12 strips) 6021 2. Chicken IgY (IgG) Std. A (0 ng/ml), 0.25 ml 6022 3. Chicken IgY (IgG) Std B (25 ng/ml), 0.25 ml 6023 4. Chicken IgY (IgG) Std C (50 ng/ml), 0. 25 ml 6024 5. Chicken IgY (IgG) Std D (100 ng/ml), 0. 25 ml 6025 6. Chicken IgY (IgG) Std E (250 ng/ml), 0. 25 ml 6026 7. Chicken IgY (IgG) Std F (500 ng/ml), 0. 25 ml 6027 8. Chicken IgY (IgG) Std G (1000 ng/ml), 0. 25 ml 6028 9. (20x) Sample Diluent, 10ml SD-20 10. Wash Buffer (100X), 10 ml WB-100 11. Anti-Chicken IgY (IgG) HRP Conjugate(100x), 0.12 ml 6029 12. TMB Substrate, 12 ml 80091 13. Stop solution, 12 ml 80101 14. Instruction Manual M-6020 SpCrossreactivity The antibodies used in this kit are specific to IgG and does not show any significant reactivity to Chicken IgA, IgM, or IgE or other serum proteins. Chicken IgG ELISA kit species cross Cross reactivity was tested with the following animal serum at dilution of 1:1k: Turkey (18%) serum showed some cross reactivity. Goose, Pigeon, Duck, FBS, Bovine, Goat, Monkey, Guinea pig, Sheep, Pig, Mouse, Rat, Human, Hamster, Bovine, and Rabbit serums all showed less than 1% cross reactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.