Alpha Diagnostic International, Inc. Mouse Anti-Jo-1 Ig's (total) ELISA Kit, 96 tests, Quantitative 6005

Mouse Anti-Jo-1 Ig's (total) ELISA Kit, 96 tests, Quantitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are a SpeciesReactivity mouse Form Stds=0-500 ng/ml; Sample=100 ul; Sensitivity=25 ng/ml; 75 min assay; Quantitative; Ag-based Ab Assay; 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Jo Coated strip plate (8 wells x 12 strips) 6006 2. Mouse anti-JO Ig?s std A ( 0 ng/ml), 0.750ml 6007 3. Mouse anti-JO Ig?s std B (31.25 ng/ml), 0.750ml. 6008 4. Mouse anti-JO Ig?s std C (62.5 ng/ml), 0.750 ml. 6009 5. Mouse anti-JO Ig?s std D (125 ng/ml), 0.750 ml. 6010 6. Mouse anti-JO Ig?s std E (250 ng/ml), 0.750 ml. 6011 7. Mouse anti-JO Ig?s std F (500 ng/ml), 0.750 ml. 6012 8. Goat Anti-mouse IgG (H+L)-HRP conjugate (100X) 0.120 ml 6013 9. Sample Diluent, (10x), 10ml SD-10 10. Wash Solution (100X), 10 ml WB-100 11. TMB Substrate solution, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual. M-6005 SpCrossreactivity Jo-1 coating antigen has been immunoaffinity purified from rabbit/calf thymus using anti-Jo-1 Sepharose. Other antigens (Sm, RNP, SSA, Scl-70, and SSB0 are not detected with the ELISA. Homogeneity of Jo-1 is also verified by SDS-PAGE.. This kit measures anti-Jo Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.