Alpha Diagnostic International, Inc. Discontinued and replaced by #6005; Mouse Anti-Jo Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative 6000

Discontinued and replaced by #6005; Mouse Anti-Jo Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Scl-70, and Jo. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Antibody to Jo antigen was originally called Jo-1. The Jo-1 antigen is histidyl-transfer ribonucleic acid synthetase, which is partially responsible for attaching t-RNA to their cognate r-RNA. Anti-Jo has been reported ~25% in polymyositis patients by immunodiffusion . The presence of anti-Jo-1 antibodies defines a distinct group of polymyositis patients with interstitial disease, arthritis, and fever. Anti-Jo-1 antibodies also inhibit the function of human histidyl tRNA synthetase. ADI?s Jo ELISA kit is a rapid, specific and sensitive assay for the detection autoantibodies to Jo-1 in mouse serum. SpeciesReactivity Mouse Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Jo Coated Microwells (96 wells) 60012. Mouse Anti-Jo negative Serum,Control 1 ml 60023. Mouse Anti-Jo positive Serum, Control 1 ml 60034. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 60045. Sample Diluent (10X), 10ml SD-106. Wash Solution (100X), 10 ml WB-1007. T MB Substrate Solution, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual M-6000 Form Stds=-ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests SpCrossreactivity Jo-1 coating antigen has been immunoaffinity purified from rabbit/calf thymus using anti-Jo-1 Sepharose. Other antigens (Sm, RNP, SSA, Scl-70, and SSB0 are not detected with the ELISA. Homogeneity of Jo-1 is also verified by SDS-PAGE..This kit detects anti-Jo Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.