Mouse Anti-SSB Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative
General Information
Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are
SpeciesReactivity
Mouse
Form
Stds= 0-75 ng/ml; Sample: 100 ul (1:100); Sensitivity; 2 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests
Samples
Serum. Plasma, Culture medium and other fluids may be adapted
KitContents
1. Purified SSB Antigen Coated (8 wells x 12 strips) 5811 2. Mouse anti-SSB Ig?s std A (0 ng/ml), 0.650 ml 5812 3. Mouse anti-SSB Ig?s std B (4.68 ng/ml), 0.650 ml 5813 4. Mouse anti-SSB Ig?s std C (9.37 ng/ml), 0.650 ml 5814 5. Mouse anti-SSB Ig?s std D (18.75 ng/ml),0.650 ml 5815 6. Mouse anti-SSB Ig?s std E (37.5 ng/ml), 0.650 ml 5 8 1 6 7. Mouse anti-SSB Ig?s std F (75 ng/ml), 0.650 ml 5 8 1 7 8. Goat Anti-Mouse IgG HRP conjugate 100X (H+L), 9. 0.120 ml 5818 10. Sample Diluent (10x), 10 ml SD-10 11. Wash Solution (100X), 10 ml 80081 12. TMB Substrate, 12 ml 80091 13. Stop solution,12 ml 80101 14. Complete Instruction Manual M-5810
SpCrossreactivity
The SSB/la antigen, used for coating ELISA plates, has been purified from calf and rabbit thymus cytosol through a combination of immunoaffinity chromatography and other biochemical techniques. The purified preparation does not contain any detectable SS-A, Sm, nRNP, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit measures anti-SSB toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
| Alpha Diagnostic International, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | 5810 |
| Product Name | Mouse Anti-SSB Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative |