Mouse Anti-SSA/Ro Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative
General Information
Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are
SpeciesReactivity
Mouse
Form
Stds= 0-4000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 125 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests
Samples
Serum. Plasma, Culture medium and other fluids may be adapted
KitContents
1. Purified SSA Antigen Coated ( 8 wells x 12 strips) 5711 2. Mouse anti-SSA Ig?s std A (0 ug/ml), 0.650 ml 5712 3. Mouse anti-SSA Ig?s std B (0.25 ug/ml),0.650 ml 5713 4. Mouse anti-SSA Ig?s std C (0.5 ug/ml), 0.650 ml 5714 5. Mouse anti-SSA Ig?s std D (1 ug/ml), 0.650 ml 5715 6. Mouse anti-SSA Ig?s std E (2 ug/ml), 0.650 ml 5716 7. Mouse anti-SSA Ig?s std F (4 ug/ml), 0.650 ml 5717 8. Goat Anti-mouse IgG (H+L)-HRP conjugate, 100X 0.120 ml 5718 9. Sample Diluent (10x), 10 ml SD-10 10. Wash Solution (100X), 10 ml WB-100 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual M-5710
SpCrossreactivity
The SS-A antigen, used for coating ELISA plates, has been purified from bovine spleen and/or thymus cytosol through a combination of immunoaffinity chromatography and other biochemical techniques. The purified preparation does not contain any detectable SS-B, Sm, nRNP, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit will measures anti-SSA toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
| Alpha Diagnostic International, Inc. | |
|---|---|
| Product Category | Biological Materials |
| Product Number | 5710 |
| Product Name | Mouse Anti-SSA/Ro Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative |