Alpha Diagnostic International, Inc. Discontinued and replaced by #5710; Mouse Anti-SSA/Ro Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative 5700

Discontinued and replaced by #5710; Mouse Anti-SSA/Ro Ig's (total (A+G+M) ELISA kit, 96 Tests, Qualitative General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. The Ro/SS-A antigen is comprised of an acidic 60 kDa protein that may exist in complex with RNA(80-112 bases). However, RNA is not required for SS-A antigenicity. Autoantibodies to SS-A antigen is found in a wide variety of connective tissue diseases but most frequently in Sjogren?s syndrome (up to 96% cases have been reported to be positive), SLE (30-50% of patients), and 83% of mothers with isolated congenital heart block or neonatal lupus dermatitis, and 75% of patients with subacute cutaneous lupus. Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis, and immunoblotting are also used to define antibody specificity. ADI?s Anti-SSA ELISA, a sandwich ELISA, provides a rapid, sensitive and semi-quantitative measurement of mouse Anti-SSA to research the development and progression of SS-A associated diseases. SpeciesReactivity Mouse Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified SS-A Coated Microwell (96 wells) 57012. Mouse Anti-SSA negative Serum Control 1 ml 57023. Mouse Anti-SSA positive Serum Control 1 ml 57034. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.12 ml 57045. Sample Diluent, (10X), 10 ml SD-106. Wash Solution (100X), 10 ml WB-1007. TMB Substrate Solution, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual M-5700 Form Stds=-ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests SpCrossreactivity The SS-A antigen, used for coating ELISA plates, has been purified from bovine spleen and/or thymus cytosol through a combination of immunoaffinity chromatography and other biochemical techniques. The purified preparation does not contain any detectable SS-B, Sm, nRNP, Scl-70, and Jo-1 antigens by sensitive ELISA technique.This kit will detect anti-SSA toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, s..