Discontinued Alternate Cat#5610 Mouse Anti-Histones Ig's (total (A+G+M) ELISA kit, Qualitative
General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Histones are a group (H1, H2a, H2b, H3, and H4) of closely related, highly basic (rich in lysine and Arginine) nuclear proteins (13-22 kDa). The basic charge on histones allows a tight association with DNA. . Anti-histone antibodies are even more common in SLE and probably account for the lupus erythematosus cell phenomenon. Anti-histone antibodies also arise as a side effect of treatment with various drugs and quite frequent in patients with drug induced lupus (DRL). Anti-histones have been found in class IV lupus nephritis (anti-H1), SLE (H1, H2b, H3), and rheumatoid arthritis with vasculitis. However, the presence of anti-histone antibodies are much more common in DRL (90-95%) than in SLE (20-30%). A large number of drugs, particularly hydralazine, procainamide, isoniazide, chlorpromazine, and methyldopa, have been associated with DRL. The phenomenon of DRL has been linked with the rate of acetylation or the activity of N-acetyltransferase. Slow acetylators are more likely to induce anti-histone antibodies than fast acetylators that can rapidly metabolize drugs and reduce drug exposure. ADI?s Anti-Histone Antibodies ELISA, has been developed to screen the presence of Anti-Histone (total) antibodies in mouse serum.
SpeciesReactivity Mouse
Samples Serum. Plasma, Culture medium and other fluids may be adapted
KitContents 1. Purified Histone Antigen Coated Microwell Strips (96 wells) 56012. Mouse Anti-Histone Negative Serum Control, 1ml 56023. Mouse Anti-Histone Positive Serum Control, 1ml 56034. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.12 ml 56045. Sample Diluent (10X), 10 ml SD-106. Wash Solution (100X), 10 ml WB-1007. TMB Substrate, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual M-5600
Form Stds= -ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests
SpCrossreactivity Purified histones used in this kit as antigen contains all bands associated with subclasses f1, f2a1, f2a2, f2b, and f3. Anti-histone antibodies, as determined by this kit, will not distinguish between these histone subisotypes. This kit will detect anti-histones toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to u..
Discontinued Alternate Cat#5610 Mouse Anti-Histones Ig's (total (A+G+M) ELISA kit, Qualitative
General Information
Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. Histones are a group (H1, H2a, H2b, H3, and H4) of closely related, highly basic (rich in lysine and Arginine) nuclear proteins (13-22 kDa). The basic charge on histones allows a tight association with DNA. . Anti-histone antibodies are even more common in SLE and probably account for the lupus erythematosus cell phenomenon. Anti-histone antibodies also arise as a side effect of treatment with various drugs and quite frequent in patients with drug induced lupus (DRL). Anti-histones have been found in class IV lupus nephritis (anti-H1), SLE (H1, H2b, H3), and rheumatoid arthritis with vasculitis. However, the presence of anti-histone antibodies are much more common in DRL (90-95%) than in SLE (20-30%). A large number of drugs, particularly hydralazine, procainamide, isoniazide, chlorpromazine, and methyldopa, have been associated with DRL. The phenomenon of DRL has been linked with the rate of acetylation or the activity of N-acetyltransferase. Slow acetylators are more likely to induce anti-histone antibodies than fast acetylators that can rapidly metabolize drugs and reduce drug exposure. ADI?s Anti-Histone Antibodies ELISA, has been developed to screen the presence of Anti-Histone (total) antibodies in mouse serum.
SpeciesReactivity
Mouse
Samples
Serum. Plasma, Culture medium and other fluids may be adapted
KitContents
1. Purified Histone Antigen Coated Microwell Strips (96 wells) 56012. Mouse Anti-Histone Negative Serum Control, 1ml 56023. Mouse Anti-Histone Positive Serum Control, 1ml 56034. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.12 ml 56045. Sample Diluent (10X), 10 ml SD-106. Wash Solution (100X), 10 ml WB-1007. TMB Substrate, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual M-5600
Form
Stds= -ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests
SpCrossreactivity
Purified histones used in this kit as antigen contains all bands associated with subclasses f1, f2a1, f2a2, f2b, and f3. Anti-histone antibodies, as determined by this kit, will not distinguish between these histone subisotypes. This kit will detect anti-histones toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to u..
