Mouse Anti-nRNP Ig's (total (A+G+M) ELISA Kit, 96 tests, Qualitative
General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are
SpeciesReactivity Mouse
Form Contact for details and availability; Quantitative; Ag-based Ab assay, 96 tests
Samples Serum. Plasma, Culture medium and other fluids may be adapted
KitContents 1. Purified Sm/nRNP Coated Microwells (96 wells) 5411 2. Mouse Anti-nRNP negative serum, control 1 ml 5412 3. Mouse Anti-nRNP positive serum, control 1 ml 5413 4. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 5414 5. Sample Diluent (10X), 10ml SD-10 6. Wash Solution. (100X), 10 ml WB-100 7. TMB Substrate Solution, 12 ml 80091 8. Stop Solution, 12 ml 80101 9. Complete Instruction Manual M-5410
SpCrossreactivity The nRNP antigen, used for coating ELISA plates, has been purified from calf and/or rabbit thymus cytosol through a combination of immunoaffinity chromatography (anti-nRNP-Sepharose
) and other biochemical techniques. The purified preparation does not contain any detectable SS-A, SS-B, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit detects anti-nRNP Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.
MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
Mouse Anti-nRNP Ig's (total (A+G+M) ELISA Kit, 96 tests, Qualitative
General Information
Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are
SpeciesReactivity
Mouse
Form
Contact for details and availability; Quantitative; Ag-based Ab assay, 96 tests
Samples
Serum. Plasma, Culture medium and other fluids may be adapted
KitContents
1. Purified Sm/nRNP Coated Microwells (96 wells) 5411 2. Mouse Anti-nRNP negative serum, control 1 ml 5412 3. Mouse Anti-nRNP positive serum, control 1 ml 5413 4. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 5414 5. Sample Diluent (10X), 10ml SD-10 6. Wash Solution. (100X), 10 ml WB-100 7. TMB Substrate Solution, 12 ml 80091 8. Stop Solution, 12 ml 80101 9. Complete Instruction Manual M-5410
SpCrossreactivity
The nRNP antigen, used for coating ELISA plates, has been purified from calf and/or rabbit thymus cytosol through a combination of immunoaffinity chromatography (anti-nRNP-Sepharose) and other biochemical techniques. The purified preparation does not contain any detectable SS-A, SS-B, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit detects anti-nRNP Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
