Alpha Diagnostic International, Inc. Mouse Anti-Sm Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative 5405

Mouse Anti-Sm Ig's (total (A+G+M) ELISA Kit, 96 tests, Quantitative General Information The Sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the Sm antigen is resistant to RNase, but partially sensitive to trypsin. It is also involved in the pathogenesis of Systemic Lupus Erythematosus (SLE). Ant SpeciesReactivity mouse Form Stds=0-1000 ng/ml; Sample=100 ul; Sensitivity=25 ng/ml; 75 min assay; Quantitative; Ag-based Ab Assay; 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified Sm Coated plate (8 wells x 12 strips) 5406 2. Mouse anti-Sm IgG std A ( 0 ng/ml), 0.750ml 5407 3. Mouse anti-Sm IgG std B (62.5 ng/ml), 0.750ml. 5408 4. Mouse anti-Sm IgG std C (125 ng/ml), 0.750 ml. 5409 5. Mouse anti-Sm IgG std D (250 ng/ml), 0.750 ml. 5410 6. Mouse anti-Sm IgG std E (500 ng/ml), 0.750 ml. 5411 7. Mouse anti-Sm IgG std F (1000 ng/ml), 0.75 ml. 5412 8. Goat Anti-mouse IgG (H+L)-HRP conjugate (100X) 0.120 ml 5413 9. Sample Diluent, (10X), 10ml SD-10 10. Wash Solution (100X), 10 ml WB-100 11. TMB Substrate solution, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual. M-5405 SpCrossreactivity This kit measure anti-Sm Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.