Alpha Diagnostic International, Inc. Discontinued Alternate Cat#5210; Mouse ANA Ig's (total (A+G+M) ELISA kit 5200

Discontinued Alternate Cat#5210; Mouse ANA Ig's (total (A+G+M) ELISA kit General Information Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases. The frequency of ANA positivity in various rheumatic diseases has been reported for SLE, rheumatoid arthritis (RA), progressive systemic sclerosis (PSS), polymyositis (PM), dermatomyositis (DM), mixed connective tissue diseases, drug-induced SLE, and Sjogren?s syndrome (SS). Most of these studies are based on tedious fluorescent ANA (FANA). Other techniques such as RIA, immunodiffusion, hemagglutination, electrophoresis, and immunoblotting are also used to define antibody specificity. Recently, immunological assays (mostly ELISA) that determine the specificity of ANA have been used in studying patients with systemic rheumatic diseases. ADI?s ANA ELISA, a sandwich ELISA, provides a rapid, sensitive and semi-quantitative measurement of mouse ANA Ig?s (IgG+A+M) to research the development and progression of ANA-associated diseases. SpeciesReactivity Mouse Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified ANA Coated Microwells (96 wells) 52012. Mouse Anti-ANA negative Serum, Control 1 ml 52023. Mouse Anti-ANA positive Serum, Control 1 ml 52034. Sample Diluent, (10X), 10 ml SD-105. Goat Anti-mouse IgG (H+L)-HRP Conjugate (100X), 0.2 ml 52046. Wash Solution (100X), 10 ml WB-1007. TMB Substrate; Solution, 12 ml 800918. Stop Solution, 12 ml 801019. Complete Instruction Manual, M-5200 Form Stds=-ve and +ve controls; Sample=100 ul (1:100); 75 min assay; Qualitative; Ag-based Ab assay, 96 tests SpCrossreactivity This kit detects ANA Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.