Alpha Diagnostic International, Inc. Mouse Anti-PCNA Ig's ELISA kit, 96 Tests, Quantitative 5010

Mouse Anti-PCNA Ig's ELISA kit, 96 Tests, Quantitative General Information Proliferating cell nuclear antigen (PCNA) protein is one of the central molecules responsible for decisions of life and death of the cell. The PCNA gene is induced by p53, while PCNA protein interacts with p53-controlled proteins Gadd45, MyD118, CR6 and, SpeciesReactivity Mouse Form Stds= 0-50 ng/ml; Sample: 100 ul (1:100); Sensitivity; 1 ng/ml; 75 min assay; Qualitative; Ag-based Ab assay; 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Purified PCNA Coated strip plate (8 wells x 12 strips). 5011 2. Mouse anti-PCNA IgG std A (0 ng/ml), 0.65 ml. 5012 3. Mouse anti-PCNA IgG std B (3.12 ng/ml), 0.65ml 5013 4. Mouse anti-PCNA IgG std C (6.25 ng/ml),0.65 ml. 5014 5. Mouse anti-PCNA IgG std D (12.5 ng/ml), 0.65 ml 5015 6. Mouse anti-PCNA IgG std E (25 ng/ml), 0.65 ml. 5016 7. Mouse anti-PCNA IgG std F (50 ng/ml), 0.65ml 5017 8. Goat Anti-mouse IgG HRP conjugate (H+L), 9. 0.120 ml , (100X) 5018 10. Sample Diluent, (10X), 10 ml SD-10 11. Wash Buffer, (100X)10 ml WB-100 12. TMB Substrate, 11 ml 80091 13. Stop solution, 11 ml 80101 14. Complete Instruction Manual. M-5010 SpCrossreactivity This kit detects anti-PCNA Ig's (IgG+A+M) in mouse serum. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.