Alpha Diagnostic International, Inc. Human IgG (total) ELISA Kit, 96 tests, Quantitative 1750

Human IgG (total) ELISA Kit, 96 tests, Quantitative General Information Immunoglobulins belong to a large group of related glyco-proteins that make up approximately 20% of the serum proteins. The serum immunoglobulins react with antigens and confer immunity to individuals. All immunoglobulins share the basic structure of: 2 SpeciesReactivity Human Form Stds=10-200 ng/ml ; Sample=100 ul; Sensitivity= 5 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum, Plasma, Culture medium KitContents 1. Anti-Human IgG coated strip plate (8 wells x 12 strips) 17512. Human IgG Std. A (0 ng/ml), 0.250 ml 17523. Human IgG Std B (25 ng/ml), 0. 250 ml 17534. Human IgG Std C (50 ng/ml), 0. 250 ml 17545. Human IgG Std D (100 ng/ml), 0. 250 ml 17556. Human IgG Std E (250 ng/ml), 0. 250 ml 17567. Human IgG Std F (500 ng/ml), 0. 250 ml 17578. Anti-Human IgG-HRP Conjugate (100x), 0.120 ml 17589. 20x Sample Diluent, 10ml SD-2010. Wash Buffer (100X), 10 ml WB-10011. TMB Substrate, 12 ml 8009112. Stop solution, 12 ml 8010113. Instruction Manual M-1750 SpCrossreactivity This kit is specific to IgG and does not show any significant reactivity to IgA, IgM, IgE or other serum proteins, however the antibodies are not specific for any IgG subtype (IgG1, IgG2, etc.). Species cross reactivity was tested with the following animal serum IgG at a concentration of 100 ug/ml: Monkey (40%), Bovine (0%) Sheep (0%), Rabbit (50%), Rat (0%), Mouse (0%), Hamster (0%) & Chicken (0%). MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.