Quantitation of dsDNA Using The 50 Microliter Volume, Re-usable and displosable FluoroVette
ALine’s FluoroVette™ (patent pending) is used for applications involving fluorescence detection. It fits into all standard fluorometers using ALine’s Universal Adapter. The design of the FluoroVette™makes it suitable for a variety of applications, including quantitation of double-stranded DNA (dsDNA). This application note details the use of the FluoroVette™ in conjunction with the fluorescent nucleic acid stain PicoGreen® for the quantitation of dsDNA in the 100-20,000pg range.
Some of the benefits for laboratories are listed below
| FluoroVette™ | Quartz Cuvette | |
| UV Transparent | 220 nm | 193 nm |
| Sample Volume | 50 µL | 50 µL |
| µVolume Capable | YES | YES |
| Disposable | YES | NO |
| Cost of Cell Washer | none | $250 |
| Maintenance (time) | none | Frequent |
| Cross Contamination | none | Possible |
For the cost of 1 quartz cuvette you can have 40 FluoroVettes™
| ALine, Inc. | |
|---|---|
| Product Category | Cuvettes |
| Product Number | Flowcell Format |
| Product Name | FluoroVette |