The OD8-DCL509-D is a dichroic beamsplitter for fluorescence filter sets, designed to reflect excitation light and transmit emission light for FITC and related fluorophores. It has a rectangular substrate measuring 25.2 x 35.6 mm with a 50% cut-on edge at 509 nm, a fused silica substrate of 1.0 mm thickness, and a clear aperture covering at least 80% of the surface. The beamsplitter achieves ≥98% average reflection across the excitation passband and ≥96% average transmission across the emission passband, with ≥98% reflection in the excitation band and ≥96% transmission in the emission band. It provides ≥OD10 average blocking within the excitation and emission bands, verified to OD8, and ≥OD4 blocking at the crossover region. The part is oriented with an arrow indicating light propagation direction and is marked with its part number on the edge.
The OD8-DCL509-D Dichroic Beamsplitter is designed to reflect the excitation and transmit the emission of FITC and supports similar alternative fluorophores, designed in conjunction with and . The filter is manufactured to provide maximum signal and sensitivity delivering exceptionally high excitation passband reflection ≥ 98% avg and emission passband transmission ≥ 96% avg for a 509 nm 50% Cut-on edge wavelength.
Alternate Fluorophores
In addition to FITC the following fluorophores are also compatible with this set:
5-FAM, 6-FAM, Abberior STAR 488, Alexa Fluor® 488, Alexa Fluor® 500, ATTO 488, ATTO 495, Azami Green, Azami Green (monomeric), BCECF (pH5.2), BCECF (pH5.5), BCECF (pH9), Calcein, CellTrace Calcein Green, CF 488A, CyQUANT®, DY-490, Emerald, Fluo-3, Fluo-4, Fluorescein (0.1 NaOH), Fluoro Emerald, FluoSpheres® Yellow-Green, HCS CellMask Green, HCS LipidTOX Green Neutral Lipid Stain, HCS LipidTOX Green phospholipidosis, HiLyte Fluor 488, LIVE/DEAD® Fixable Green Dead Cell Stain, LysoTracker® Green, mAzamiGreen, mHoneydew, MitoTracker® Green, MitoTracker® Green FM, mWasabi, NBD, NBD-X (MeOH), NeuroTrace® 500/525 Green Fluorescent Nissl Stain (RNA), Oregon Green® 488, PA-GFP (post-activation), PicoGreen® Reagent, PromoFluor-488, Rhodamine 110, Rhodamine Green, Rhodol Green, Spectrum Green, SYBR® Gold, SYBR® Green I, SYTO® 11, SYTO® 13, SYTO® 16, SYTO® RNASelect, SYTOX® Green, Vybrant® DyeCycle Green, ZsGreen1.
Optimized Coatings and Engineering Expertise
Newport pushed the boundaries with the introduction of our ODiate Fluorescence Filter Sets, to allow customers to fully optimize their system performance. Our expert engineering team has been involved from the first stage of design to ensure that we exceed your requirements and continues through the entire process of continual production deliveries.
Environmental Stability and Physical Durability
The coating systems used to produce ODiate filters use a dense sputtering process to provide robust environmental reliability to meet the highest demands for thin-film coatings. The resulting highly durable coatings meet or exceed testing procedures including humidity, thermal shock, adhesion, chemical resistance, and moderate abrasion per U.S. Military and international standards.
| Newport MKS | |
|---|---|
| Product Category | Polarizing Cube Beamsplitters |
| Product Number | OD8-DCL509-D |
| Product Name | ODiate® Dichroic Beamsplitter, 25.2 x 35.6 mm, 509 nm 50% Cut-on Edge Wavelength |
| Side Length | 35.6 mm (1.4 inch) |
