Life Sciences, Inc. AMV Reverse Transcriptase in Trehalose AMVRTT-5

Applications Whole serum: Purified protein p27 from AMV is isolated at LSAT by column chromatography as a by-product generated during isolation of AMV Reverse Transcriptase from avian myeloblastosis virus. This protein is used as an antigen to raise high-titered polyclonal antibodies in rabbit antisera. IgG Fraction: Hyperimmune serum is fractionated using ammonium sulfate precipitation and anion exchange chromatography. This product is particularly suited for coating microtiter plates for use in a direct ELISA assay. Supplied in phosphate buffered saline, pH 7.2 at a concentration of 5.0 mg per mL. (Quantitation performed by UV absorption, using an absorption coefficient of 1.38 A280 units per concentration unit of 1.0 mg per mL). Horseradish peroxidase conjugated IgG: IgG from high-titered hyperimmune serum is conjugated to horseradish peroxidase Type VI. When diluted at either 1:2000 or 1:5000 this conjugate is capable of detection of 1 ng of p27 protein. Concentration of protein is 3.6 mg/mL. General Notes A direct ELISA protocol, in which IgG is used to coat microtiter plates, may be used to detect p27 antigen released by viral lysis from samples such as plasma from viremic chickens, and albumen from infected eggs. One mL of IgG (AP2705) may be used to coat from 50 – 75 ninety-six well microtiter plates. Customer Inquiries Bulk quantities of our immunological products are offered at discounted prices! Please inquire. Life Sciences Advanced Technologies, Inc. desires to make its products easily available to the chicken industry worldwide. If you would be interested in obtaining these products in lyophilized form, (which means less expensive shipping and greater product stability), please indicate your interest by e-mail.