Ready-to-use affinity media kit, includes 5 ml Affi-Gel protein A support, Affi-Gel Protein A MAPS II buffers, 1 x 10 cm Econo-Column column; for 500 mg of mouse IgG1

Chromatography on Affi-Gel and Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgG from mammalian species.
Advantages include:
In addition, Affi-Prep media offer high linear flow rates up to 2,000 cm/hr, pressure stability up to 1,000 psi (70 bar), and high chemical stability, which allows sanitization with 0.1 M NaOH.
With Affi-Gel and Affi-Prep protein A media and MAPS II buffers, you can purify up to 10 mg of IgG1/ml of gel. This is 8–10 times higher than with standard methods. In addition, the MAPS process and Affi-Gel protein A permit greater binding of mouse IgG1 than do immobilized protein G media.
| Bio-Rad Laboratories, Inc. | |
|---|---|
| Product Category | Gel Electrophoresis Equipment |
| Product Number | 153-6159 |
| Product Name | Affi-Gel Protein A MAPS II Kit |