Human Free T4 (fT4) ELISA Kit, 96 tests, Quantitative
General Information Thyroxine (T4), the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. However, only the free (unbound) fraction of thyroxine is considered to be biologically active. The main carriers of thyroxine are thyroxine-b
KitContents Please refer to complete instructions manual supplied with the product
SpeciesReactivity Human
Form Stds=0-100 pg/ml ; Sample=25 ul; Sensitivity= 1 pg/ml; 75 min assay; Quantitative; Competitive assay; 96 tests
Samples Serum. Other fluids not optimized.
SpCrossreactivity The following compounds were tested for cross-reactivity with the Direct fT4 ELISA kit with T4 cross-reacting at 100%: L-Thyroxine (100%), D-Thyroxine (94%), 3,3?,5?-Triiodo-L-Th
yronine (Reverse T3) (86%), 3,3?,5-Triiodo-L-Thy
ronine (T3) (3.3%), 3,3?,5?-Triiodo-D-Th
yronine (1.8%), 3,3?,5?-Triiodothyro
propionic acid (0.6%). The following compounds were tested but cross-reacted at less than 0.04%: Acetylsalicylic acid, 3,5-Diiodo-L-Thyroni
ne, 3,5-Diiodo-L-Tyrosin
e and 3-Iodo-L-Tyrosine. T4 is the same in human and animals. ADI human T4 elisa Kit has been used in mouse and rat samples (see publications).
MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
Human Free T4 (fT4) ELISA Kit, 96 tests, Quantitative
General Information
Thyroxine (T4), the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. However, only the free (unbound) fraction of thyroxine is considered to be biologically active. The main carriers of thyroxine are thyroxine-b
KitContents
Please refer to complete instructions manual supplied with the product
SpeciesReactivity
Human
Form
Stds=0-100 pg/ml ; Sample=25 ul; Sensitivity= 1 pg/ml; 75 min assay; Quantitative; Competitive assay; 96 tests
Samples
Serum. Other fluids not optimized.
SpCrossreactivity
The following compounds were tested for cross-reactivity with the Direct fT4 ELISA kit with T4 cross-reacting at 100%: L-Thyroxine (100%), D-Thyroxine (94%), 3,3?,5?-Triiodo-L-Thyronine (Reverse T3) (86%), 3,3?,5-Triiodo-L-Thyronine (T3) (3.3%), 3,3?,5?-Triiodo-D-Thyronine (1.8%), 3,3?,5?-Triiodothyropropionic acid (0.6%). The following compounds were tested but cross-reacted at less than 0.04%: Acetylsalicylic acid, 3,5-Diiodo-L-Thyronine, 3,5-Diiodo-L-Tyrosine and 3-Iodo-L-Tyrosine. T4 is the same in human and animals. ADI human T4 elisa Kit has been used in mouse and rat samples (see publications).
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
