Human Total Thyroxine (T4) ELISA Kit, 96 tests, Quantitative
General Information The thyroid gland produces T4, trilodothyronine T3 and calcitonin. The first two hormones are synthesized by the gland following entrapment of iodine, conversion to iodine, and coupling of iodine with tyrosine, followed by coupling of two iodinated tyros
KitContents Please refer to complete instructions manual supplied with the product
SpeciesReactivity Human
Form Stds=0-25 ug/dL; Sample=25 ul; Sensitivity= 0.5 ug/dL; 90 min assay; Quantitative, Competitive assay
Samples Serum. T4 assay in Plasma (EDTA) have also been used (see published references). Other fluids may be adapted
SpCrossreactivity The specificity of total T4 ELISA kit was determined by measuring interference from high concentrations of 3,5-diiodothyronine (up to 10 ug/dL), 3,3',5-triiodothyron
ine (rT3,,up to 1000 ng/ml), 3,3',5-triiodothyron
ine (T3,,up to 59 nmol/l), 3,3',5-triiodothyroa
cetic acid (up to 42 nmol/l), 3,3',5-triiodothyrop
ropionic acid (up to 63 nmol/l), Aspirin (10 mg/dL) iodoacetic acid (10 ug/dL), phenylbutazone (10 mg/dL). No significant cross-reaction was observed with any of these compounds. T4 is the same in human and animals. ADI human T4 elisa Kit has been used in mouse and rat samples (see publications).
MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
Human Total Thyroxine (T4) ELISA Kit, 96 tests, Quantitative
General Information
The thyroid gland produces T4, trilodothyronine T3 and calcitonin. The first two hormones are synthesized by the gland following entrapment of iodine, conversion to iodine, and coupling of iodine with tyrosine, followed by coupling of two iodinated tyros
KitContents
Please refer to complete instructions manual supplied with the product
SpeciesReactivity
Human
Form
Stds=0-25 ug/dL; Sample=25 ul; Sensitivity= 0.5 ug/dL; 90 min assay; Quantitative, Competitive assay
Samples
Serum. T4 assay in Plasma (EDTA) have also been used (see published references). Other fluids may be adapted
SpCrossreactivity
The specificity of total T4 ELISA kit was determined by measuring interference from high concentrations of 3,5-diiodothyronine (up to 10 ug/dL), 3,3',5-triiodothyronine (rT3,,up to 1000 ng/ml), 3,3',5-triiodothyronine (T3,,up to 59 nmol/l), 3,3',5-triiodothyroacetic acid (up to 42 nmol/l), 3,3',5-triiodothyropropionic acid (up to 63 nmol/l), Aspirin (10 mg/dL) iodoacetic acid (10 ug/dL), phenylbutazone (10 mg/dL). No significant cross-reaction was observed with any of these compounds. T4 is the same in human and animals. ADI human T4 elisa Kit has been used in mouse and rat samples (see publications).
MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
For research use only, not for diagnostic or therapeutic use.
