Alpha Diagnostic International, Inc. Mouse C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative 1040

Mouse C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative General Information C-reactive protein (CRP) has been regarded as an acute phase reactant in serum (1). It consists of five single subunits, which noncovalently linked and assembled, as a cyclic pentamer with a mol. Wt. Range of 110-140 kDa (2). CRP has been found to be in SpeciesReactivity mouse Form Stds=0-250 ng/ml ; Sample=100 ul (1;100) Sensitivity= 2 ng/ml; 125 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Plasma, Culture medium and other fluids may be adapted KitContents 1. Anti-Mouse CRP coated strip plate ( 8 wells x 12 strips) 1041 2. Mouse CRP Reference Standard, lyophilized Reconstitute with 0.200 ml distilled water 1042 3. Anti-Mouse CRP-HRP Conjugate, 11 ml CRP-HRP 4. 1x Sample Diluent, 30 ml CRP-SD 5. 20x Wash Buffer, 50 ml WB-20 6. TMB Substrate, 11 ml TMB-CRP 7. Stop solution, 11 ml CRP-S 8. Instruction Manual M-1040 SpCrossreactivity Mouse CRP ELISA kit species cross reactivity was tested with the following CRP proteins at 100 ng/ml: Rat CRP showed >200% reactivity, and human CRP showed ~10% reactivity. Monkey, Rabbit and dog CRP all showed less that 1% cross reactivity. The following serums showed less than 1% cross reactivity when diluted at 1:100: Bovine, FBS, Hamster, Guinea Pig, Rat, Sheep, Chicken, Pig, and Goat. ADI provides CRP ELISA kits For Human, Mouse, Rat, Monkey, Rabbit and Dog. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.