Alpha Diagnostic International, Inc. Rat C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative 1010

Rat C-Reactive Protein (CRP) ELISA Kit, 96 tests, Quantitative General Information -reactive protein (CRP) has been regarded as an acute phase reactant in serum (1). It consists of five single subunits, which noncovalently linked and assembled, as a cyclic pentamer with a mol. Wt. Range of 110-140 kDa (2). CRP has been found to be inc SpeciesReactivity Rat Form Stds=2.5-60 ng/ml ; Sample=100 ul (diluted); Sensitivity= 2 ng/ml; 105 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Rat CRP assay in Plasma (EDTA), culture medium (VSMC cells) have also been used (see published references). Other fluids may be adapted KitContents 1. Rabbit Anti-Rat CRP IgG coated strip plate ( 8 wells x 12 strips) 1011 2. Rat CRP Std. A (0 ng/ml) 0.2 ml 1012 3. Rat CRP Std. B (12.5 ng/ml) 0.2 ml 1013 4. Rat CRP Std. C (25 ng/ml) 0.2 ml 1014 5. Rat CRP Std. D (100 ng/ml) 0.2 ml 1015 6. Rat CRP Std. E (300 ng/ml) 0.2 ml 1016 7. 100x Anti-rat CRP-HRP Conjugate, 0.12 ml 1017 8. 100x Wash Buffer, 10 ml WB-100 9. 20x Sample Diluent, 10ml SD-20 10. TMB Substrate, 12 ml 80091 11. Stop solution, 12 ml 80101 12. Instruction Manual M-1010 SpCrossreactivity Serum samples from mouse, human, pig, goat, FBS, Bovine, G. pig, hamster, monkey, and Chicken showed less than 1% crossreactivity with rat CRP ELISA kit at dilutions of 1:400. Rabbit serum showed ~10% cross reactivity. ADI provides human CRP ELISA kit cat # 1000 for detecting human serum CRP. ELISA kits for mouse, rat, human, monkey, rabbit CRP are also available. Rat IgG, IgM, and other serum protein ELISA kits can be obtained from ADI. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.