Alpha Diagnostic International, Inc. Human Beta-2 microglobulin (B2M) ELISA Kit, 96 tests, Quantitative 0020

Human Beta-2 microglobulin (B2M) ELISA Kit, 96 tests, Quantitative General Information Human Beta-2 Microglobulin (B2M) is a small (11.0 kDa) polypeptide that forms the invariant subunit of the class I HLA-antigens (Human Lymphocyte Antigen) on the cell membrane (1). Increased concentrations of B2M in serum have been found in patients with KitContents Please refer to complete instructions manual supplied with the product SpeciesReactivity Human Form Stds=0.75-12 ug/ml ; Sample=10 ul; Sensitivity= 0.1 ug/ml; 60 min assay; Quantitative, Sandwich, 96 tests Samples Serum. Other fluids not optimized but may be tested. SpCrossreactivity Human Beta-2 microglobulin (B2M) ELISA Kit crossreactivity with B2M from other species was determined by testing sera from various species. Bovine, Cat, Dog, Donkey, Hamster, Horse, Mouse, Rat B2M showed no significant crossreactivity was detected using 1:5 diluted sera. At 1:5 dilution monkey sera yielded values equal to or higher than the equivalent human serum. It is concluded that the human kit has equal reactivity with the monkey beta-2 microglobulin. Due to the lack of purified B2M or known standards, it is not possible to determine absolute crossreactivity. MSDS TMB (substrate), H2SO4 (1% in a buffered stop solution), and Proclin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates For research use only, not for diagnostic or therapeutic use.